light microscopy  (Carl Zeiss)

Journal: Cell Stress & Chaperones

Article Title: Dynamics of heat shock protein 70 kDa in heat-shocked and hypoxic human endothelial cells

doi: 10.1016/j.cstres.2025.100085

Figure Lengend Snippet: Morphological characterization of thermal stress in human endothelial cells. (a) Primary HUVECs were exposed to heat shock (HS) at 42 °C for 1 h and allowed to recover at 37 °C for 24 h. Images were acquired using optical microscopy (ZEISS Primovert) before HS (0 h), immediately after HS (1 h), and after recovery (24 h). Scale bar: 100 µm. Inset scale bar: 50 µm. Representative images of n = 8 independent experiments using cells from different lots (19TL028325, 19TL023264, 19TL127368, and 21TL195720, Lonza). (b) Supernatants from these cells were collected, and HS cytotoxicity was measured by quantifying lactate dehydrogenase (LDH) activity. The data are shown as the means ± SEMs of 3 independent experiments (lot 19TL127368 and 19TL028325), ordinary one-way ANOVA, with Tukey’s multiple comparisons test, ** P < 0.01, *** P < 0.001 versus positive control. HUVECs were treated with Triton X-100 to achieve maximum LDH release from the supernatant. (c) HSF1 (green, CST 4356S) and total HSP70 (red, Invitrogen MA3-006). Abbreviation used: HUVEC, human vein.

Article Snippet: Fig. 1 Fig. 2 Morphological characterization of thermal stress in human endothelial cells. (a) Primary HUVECs were exposed to heat shock (HS) at 42 °C for 1 h and allowed to recover at 37 °C for 24 h. Images were acquired using optical microscopy (ZEISS Primovert) before HS (0 h), immediately after HS (1 h), and after recovery (24 h).

Techniques: Microscopy, Activity Assay, Positive Control